Highly Refractory Myeloma Has Deep and Durable Responses With Ide-cel

Patients with heavily pretreated multiple myeloma maintained durable responses with the chimeric antigen receptor (CAR) T-cell therapy idecabtagene vicleucel (ide-cel; bb2121) in updated findings presented from the phase 1 CRB-401 trial (NCT02658929).

The objective response rate (ORR) in the entire treated patient population (N = 62) was 75.8% with a rate of complete response (CR) or greater of 38.7%. All 15 patients who achieved a CR or greater and had bone marrow evaluable for minimal residual disease (MRD) demonstrated MRD negativity by next-generation sequencing. 

“Patients with myeloma who have been treated with the 3 main backbones of interventional therapy—proteasome inhibitors [PIs], IMiDs [immunomodulatory drugs], and monoclonal antibodies—have poor outcomes to subsequent treatment. This includes a median PFS [progression-free survival] seen to date of less than 6 months and a median OS [overall survival] of less than 1 year,” Yi Lin, MD, PhD, said during a presentation at the 62nd American Society of Hematology (ASH) Annual Meeting and Exposition. “With longer follow-up, we are continuing to see a deep response with ide-cel in this heavily pretreated myeloma patient population.”

Ide-cel is manufactured from patients T cells using a lentiviral construct, which encodes a CAR specific for human B-cell maturation antigen (BCMA) with a 4-1BB costimulatory domain and a CD3V T-cell activation domain. Lin, who is an assistant professor of oncology and medicine at the Mayo Clinic in Rochester, Minnesota, noted that manufacturing success was 100% on this study.

Following leukapheresis, patients received fludarabine 30 mg/m2 plus cyclophosphamide 300 mg/m2 on days –5 to –3 prior to CAR T-cell infusion. In the dose-escalation portion of the study, patients with 3 or more prior lines of therapy (n = 21), including a PI and an IMiD, were administered between 50 × 106 and up to 800 × 106 CAR-positive T cells. In the dose-expansion portion, additional patients were administered 150 × 106 (n = 12) or 450 × 106 (n = 29) CAR-positive T cells. The criteria for inclusion in the dose-expansion phase included having 3 or more prior lines of therapy, including a PI, IMiD, and daratumumab (Darzalex), and being refractory to the last line of therapy. As of the median follow-up of 18.1 months, 11 patients (17.7%) were still ongoing on the study.

The primary end point of the study was safety and the secondary end point was ORR.

Investigators found a dose-related increase in response rate. Patient treated with the 50 × 106 dose (n = 3) had an ORR of 33.3%, composed of 1 partial response (PR). Those receiving the 150 × 106 dose (n = 18) had an ORR of 50.0%, composed of mostly CRs (5.6%) or stringent CRs (sCR; 33.3%). In the 450 × 106 dose cohort (n = 38), the ORR was 89.5% with 31.6% sCRs, 5.3% CRs, 42.1% very good PRs (VGPRs), and 10.5% PRs. All 3 patients treated with the 800 × 106 dose responded, 1 with an sCR and 2 with CRs.

Duration of response (DOR) was also considered dose related, and the median for all dosed patients was 10.3 months (95% CI, 7.7-13.7). The median DOR was greatest in those with a CR or better at 14.8 months (95% CI, 8.1-21.5), followed by VGPRs at 9.2 months (95% CI, 3.0-10.3), then PRs at 4.6 months (95% CI, 1.9-12.5). Half of the patients with ongoing responses (n = 8) have DORs greater than 2 years.

DOR was assessed based on poor prognostic factors with investigators reporting no correlation between factors such as increased age, high Revised International Staging System stage, extramedullary plasmacytoma, or the receipt of bridging therapy and DOR.

Median PFS was 8.8 months (95% CI, 5.9-11.9) across all treated patients. Median OS was 34.2 months (95% CI, 19.2-not evaluable).

Lin reported that ide-cel expansion appeared to peak within the first month of administration and remained persistent and detectable many months after infusion. For those reaching a CR or better, peak expansion of CAR T cells was higher than in those who did not.

Patient baseline characteristics revealed a heavily pretreated population with 6 median prior lines of therapy (range, 3-18), close to 70% of whom were refractory to PIs, IMiDs, and anti-CD38 monoclonal antibodies. High-risk cytogenetics were present in 27.4% of patients and extramedullary disease was recorded in 37.1%. About half (51.6%) of these patients required bridging therapy during manufacturing of CAR T-cell therapy. The median age of all patients was 61 years (range, 37-75) and the majority had an ECOG performance status of 1 (71.0%).

The most common any-grade and grade 3/4 adverse effects observed were cytopenias. Neutropenia of any grade occurred in 91.9% of patients, anemia in 75.8%, thrombocytopenia in 74.2%, leukopenia in 64.5%, and lymphopenia in 37.1%. Corresponding rates of grade 3/4 cytopenias were 88.7%, 56.5%, 56.5%, 61.3%, and 35.5%, respectively. In patients without blood count recovery within 1 month, median recovery times for neutropenia and thrombocytopenia were 1.9 months and 2.2 months, respectively.

One death (1.6%) within 8 weeks of CAR T-cell infusion was reported in a patient who had grade 2 cytokine release syndrome (CRS) events on days 1 and 8, resolving on days 4 and 12, respectively. The patient was found to have a minimal response by IMWG criteria at month 1 and had persistent cytopenia requiring transfusions. The patient withdrew from the study and died in hospice of unknown cause at day 51. Seven more deaths (11.3%) occurred within 6 months of CAR T-cell infusion, 6 of which were due to myeloma progression and 1 due to cardiopulmonary arrest that was determined to not be treatment related.

In general, increased ide-cel doses were associated with higher rates of CRS. However, the majority of cases were mild, with only 6.5% of patients having grade 3 events. The median time to first onset of CRS was 2 days (range, 1-19) and the median duration was 5 days (range, 1-32). Tocilizumab (29.0%) and corticosteroid (16.1%) rates were relatively low for management of CRS in this population.

The majority of neurologic events were grade 1 (27.4%) with a median duration of 3 days (range, 1-62) and a median onset of day 5 (range, 1-30). Corticosteroids were used to control neurologic events in 8.1% of patients.

“These results so far are comparable to those results reported by [Nikhil C. Munshi, MD,] at ASCO [American Society of Clinical Oncology Virtual Scientific Program] earlier this year of the pivotal trial of KarMMa [NCT03361748] in this patient population,” said Lin.

The phase 2 trial presented in May revealed an ORR of 73%, with a CR rate of 33%, in patients with triple-class refractory multiple myeloma.2 The median DOR was 10.7 months in patients who were treated with ide-cel doses between 150 × 106 and 450 × 106.

Ide-cel is also being explored in multiple other studies, including the phase 3 randomized trial versus standard regimens in patients who are triple-class exposed with between 2 and 4 prior lines of therapy (KarMMa-3; NCT03651128).

References

1. Lin Y, Raje NS, Berdeja JG, et al. Idecabtagene Vicleucel (ide-cel, bb2121), a BCMA-Directed CAR T Cell Therapy, in Patients with Relapsed and Refractory Multiple Myeloma: Updated Results from Phase 1 CRB-401 StudyPresented at: 62nd American Society of Hematology (ASH) Annual Meeting and Exposition; December 5-8, 2020; Virtual. Abstract 131.
2. Munshi NC, Anderson LD, Shah N, et al. Idecabtagene vicleucel (ide-cel; bb2121), a BCMA-targeted CAR T-cell therapy, in patients with relapsed and refractory multiple myeloma (RRMM): Initial KarMMa results. J Clin Oncol. 2020;38(suppl 15):8503. doi:10.1200/JCO.2020.38.15_suppl.8503